Authors : Zhang Jing-Hu, Gong Wei-Hua and Yang Hong-Wen
Abstract: The full-length cDNA sequence of one goat gene RHOA was amplified using the rapid amplification of cDNA ends (RACE) method based on one goat EST sequence which was highly homologous to the coding sequence of human RHOA gene. Sequence prediction analysis revealed that the open reading frame of this gene encodes a protein of 193 amino acids that shares high homology with the ras homolog gene family, member A (RHOA) of nine species human (100%), cattle (100%), sheep (100%), rat (99%), mouse (99%), chicken (99%), dog (99%), turkey (99%) and green anole (99%) so that it can be defined as goat RHOA gene. This novel goat gene was then deposited into NCBI database. Phylogenetic analysis revealed that the goat RHOA gene has a closer genetic relationship with the RHOA genes of human, cattle and sheep. Tissue expression analysis indicated that the goat RHOA gene is generally and differentially expressed in detected tissues including spleen, muscle, skin, kidney, lung, liver, fat and heart. This experiment established the primary foundation for further research on the goat RHOA gene.
Zhang Jing-Hu, Gong Wei-Hua and Yang Hong-Wen, 2012. Isolation, Sequence Identification and Tissue Expression Profile of a Novel Goat Gene RHOA. Agricultural Journal, 7: 194-197.