Abstract: This study aimed to determine the relationship between organizational climate and job stress of faculty members in Ferdowsi University of Mashhad. The current study objectively is applied research and in terms of nature and method is descriptive-correlation survey. The study population consisted of all faculty members of Ferdowsi University of Mashhad. The sample size was estimated 250 people according to the formula of determining sample size. Sampling method is stratified random sampling that using proportional allocation formula, the participants were selected. Standardized organizational climate questionnaire of Kolb; Rubin and Mcintyre and also job stress questionnaire of Ivancevich and Matteson was used for collecting the field data. Questionnaires reliability and validity were investigated and confirmed. To test the hypotheses, Pearson-correlation coefficient test method is used. The research results showed that, there is a significant inverse relationship between organizational climate and job stress of faculty members. Moreover, there is a significant inverse relationship between organizational climate variables such as; responsibility, standards, rewards, organization clarity, warmth and support, leadership with job stress. In the end, the required proposals are presented to improve organizational climate and to minimize job stress of faculty members.