Authors : Siddig Ibrahim Abdel-Wahab , Ahmad Bustamam Abdul , Hew Kim Fong , Syam Mohan , Manal Mohamed Elhassan , Adel Sharaf Al-Zubairi and Abdelbasit Adam Mariod
Abstract: The aim of the present study, is to evaluate the antimicrobial, phenolic content and free radical scavenging properties of the dichloromethane extract of Goniothalamus umbrosus leaves. The antimicrobial activities were evaluated using 2 Gram-positive bacteria, Methicillin Resistant Staphylococcus aureus (MRSA) and Bacillus subtilis B 29 and 2 Gram-negative bacteria, Pseudomonas aeruginosa 60690 and Salmonella choleraesuis using disc diffusion method and compared alongside to streptomycin. Antioxidant effect and total phenolic content of the extract were also measured by DPPH assay and Folin-Ciocalteu reagent, respectively. The results have concluded that the extract explicit a broad spectrum antimicrobial activities against all tested bacteria. However, antioxidant activity is significantly different from the activity of the positive control, BHT. Accordingly, the total phenolic compounds were also, observed to be correlated positively with the low antioxidant activity revealed by the extract. As a conclusion, the promising broad spectrum antimicrobial activities of the dichloromethane extract of the leaves of G. umbrosus might be due a different chemical constituent (s) since, the phenolic compounds were not found richly in the extract. Further, phytochemical investigations are currently conducted to explore the active ingredients as new substance (s) in the pharmaceutical industry.
Siddig Ibrahim Abdel-Wahab , Ahmad Bustamam Abdul , Hew Kim Fong , Syam Mohan , Manal Mohamed Elhassan , Adel Sharaf Al-Zubairi and Abdelbasit Adam Mariod , 2009. Antimicrobial and Free Radical Scavenging Activities of the Dichloromethane Extract of Goniothalamus umbrosus. International Journal of Tropical Medicine, 4: 32-36.