Authors : Terwase Fabian Ikpa and Adesoji Adedapo Adebambo
Abstract: The response of Immunoglobulin G (IgG) subclass to Plasmodium falciparum Circumsporozoite Protein (CSP)-R32tet32 was evaluated among febrile Nigeria children aged 2-10 years (n = 23) by the Enzyme Linked Immunosorbent Assay (ELISA). The mean absorbance of normal IgG subclass and anti-CSP specific antibody of each subclass was negatively correlated and was significant for IgG3 (r = - 0.358, p = 0.05) and IgG4 (r = - 0.403, p = 0.03). The predominant anti-CSP antibodies were IgG1 and IgG3, co-expressed in nearly 60% of the subjects. Cytophilic IgG1 and IgG3 were associated with positive recognition of the CSP antigen (Fisher’s exact probability test: p = 0.0001). Some 34.78% of the children did not produce any anti-CSP specific antibodies despite previous exposure to sporozoite inoculation. This study demonstrates that cytophilic IgG subclasses are the main antibodies produced against the CSP but some children living in a holoendemic malaria transmission area may not produce anti-CSP specific antibodies. There is a need to investigate the antibody response of this group of children with the CSP based RTS, S/AS vaccine candidate molecules.
Terwase Fabian Ikpa and Adesoji Adedapo Adebambo, 2011. Immunoglobulin G Subclass Responses to Plasmodium falciparum Circumsporozoite Protein among Nigerian Children. International Journal of Tropical Medicine, 6: 100-105.