Authors : Salah M. M. Elamin , Salah H. Idris , Mohammed M. salih and Rihab A. Omer
Abstract: A reverse transcriptase polymerase chain reaction (RT-PCR) protocol was evaluated for detection of bluetongue virus ribonucleic acid in cell culture. BTV serotypes 1, 2, 4, 5, 10, 11, 13, 16 and 17 were studied. RNAs from these BTV serogroup, propagated in cell cultures, were detected by the described RT-PCR-based assay. The specific 519 bp PCR products were visualized on ethidium bromide-stained agarose gel using a pair of primers derived from segment 6 of BTV 11. Amplification product was not detected when the RT-PCR-based assay was applied to RNA from epizootic hemorrhagic disease virus (EHDV) or palyam virus serogroups; or total nucleic acid extracts from uninfected Vero cells. The results of this study indicated that the described RT-PCR assay could be applied for detection of BTV serogroup. In addition, the described BTV RT-PCR assay could be used as a supportive diagnostic assay to the current conventional virus isolation procedures used for detection of BTV in cell cultures.
Salah M. M. Elamin , Salah H. Idris , Mohammed M. salih and Rihab A. Omer , 2004. Detection of Bluetongue Virus Serogroup in Cell Culture Using RT-PCR . Journal of Animal and Veterinary Advances, 3: 754-757.