Abstract: In Sari (north of Iran), in spite of abundant presence of stray cats, no human infection caused by M. canis have been reported yet. The aim of this study was to evaluate the frequency of fungal flora health carriers among the stray cats in Sari and to determine the prevalence of M canis carrier status in population sampled. One hundred stray cats (n=100) were captured in different parts of Sari. Age, gender and Cutaneous lesions were recorded. All cats were free of skin diseases. The specimens were obtained from coat using a sterile toothbrush. Sabouraud Dextrose Agar (SDA), Mycosel agar (SCC) and Dermatophytes Test Medium (DTM) were used for initial fungal isolations. Plucked hairs and scraped scales were examined in 10% Potassium Hydroxide (KOH) with or without 0.1% calcofluor white (KOH+CFW) for fungal elements. Seventy seven cats (77%) were adult and 23 (23%) were kittens. Fifty-six cats (56%) were male and 44 cats (44%) were female. All cats were clinically normal. Eleven identifiable genera of fungi were isolated from these cats. Nine of the genera are commonly considered as saprophytes. M.gypseum was isolated in 3 cats and Trichophyton mentagrophytes Var. mentagrophytes in one. M. canis was not isolated from the samples under study. The most frequent saprophytic fungal isolates were Aspergillus, Cladosporium, Alternaria, Penicillium, Mucor, Rhizopus and Scopulariopsis sp. It was found that, 10% KOH-0.1% CFW preparation has higher sensitivity than 10% KOH preparation test. Normal fungal flora of the coat of stray cats is principally composed of saprophytic fungi, most likely seeded from the environment and that Stray cats are not a significant reservoir of M. canis in Sari.