Authors : M.D Achukwi, D. Berthier , I. Chantal and J.C. Maillard
Abstract: Genomic DNA samples were obtained from blood buffy coat preparations of Namchi or Doayo (Bos taurus) and Akou or White Fulani (Bos indicus) cattle that were randomly chosen from several herds in the region of Poli, Cameroon for the purpose of identifying hitherto unknown BoLA DQB alleles. The DQB (207 bp) exon 2 fragments were amplified by Polymerase Chain Reaction (PCR). The PCR products were cloned with the Promega pGEMT-T Vector System II kit. Sequencing of the DQB exon 2 clones was performed on an Applied Biosytems 377 automated sequencer using 3’ dye labelled dideoxynucleotide triphosphate (Dye terminator). After verification of the forward and reverse sequences of each allele in six clones of at least three different animals, the amino acid sequences were compared with all the DQB alleles referenced in the official BoLA Nomenclature (http://www2.ri.bbsrc.ac.uk/bola/). The new BoLA DQB exon 2 sequences were assigned allele names by the BoLA nomenclature committee of the International Society for Animal Genetics. In all six new BoLA-DQB alleles from Namchi/Doayo and Akou White Fulani cattle were sequenced and given the accession numbers indicated in brackets: CIR1 (AJ421631), CIR2 (AJ421632), CIR3 (AJ421633); CIR4 (AJ421634), CIR5 (AJ421635) and CIR6 (AJ421636). CIR1, CIR2 and CIR3 were identified in Doayo and Akou cattle, CIR4 and CIR5 occurred only in Doayo while CIR6 (AJ421636) occurred only in Akou cattle. Breed-specific alleles may be indicators of certain breed-specific characteristics such as disease resistance or susceptibility; so this needs to be investigated further.
Achukwi, M.D , D. Berthier , I. Chantal and J.C. Maillard , 2006. Six New Bola-DQB Alleles Sequenced from Doayo/Namchi (Bos taurus) and Akou/White Fulani (Bos indicus) Cattle. Journal of Animal and Veterinary Advances, 5: 426-429.