Abstract: The objective of the present study was to evaluate the treatment of Persistent Mating Induced Endometritis (PMIE) in Arabian maiden mares using uterine lavage containing antibiotics alone or with uterotonic agents. Maiden mares (n = 42) with PMIE were subjected to detailed clinical examinations including palpation per rectum, vaginoscopy and cytological examination. The mares were qualified as PMIE due to accumulation of uterine fluid 1-2 days post breeding. Uterine lavage containing crystalline penicillin and streptomycin sulphate was performed (n = 36) 6 h post-breeding. After 8 h of mating maiden mares were randomly divided into 3 groups, 12 animals in each group. The first group injected with 250 �g of cloprostenol (PGF2 ) S.C. and the second group received oxytocin 20 I.U. I.M., while the third group with no hormonal treatment. Control maiden mares (n = 6) received neither uterine lavage nor hormonal preparations. Results of pregnancy rate revealed 83.3% pregnancy of maiden mares treated with uterine lavage and PGF2 and 66.6% in mares treated with uterine lavage and oxytocin. In a conclusion, maiden mares with PMIE could be successfully treated with uterine lavage and PGF2 .