Authors : Aboutorab Tabatabaei Naeini , Ahmad Oryan , Seifollah Dehghani and Behrooz Nikahval
Abstract: The effects of local and parenteral phenytoin therapy in the experimentally induced cutaneous wounds in rabbits are investigated by light and electron microscopy, biomechanical investigation and the extent of wound closure. Thirty skeletally mature rabbits were randomly divided into 2 experimental groups and a control group (each group n = 10). Under sterile conditions, surgical skin incisions were made perpendicular to the vertebral column in both sides of the paralumbar region. On left side only the skin was removed (superficial wound) but on the right side, the skin and the underlying tissues including subcutaneous fascia, panniculus carnosus were also excised (deep wound). One group received daily topical application of phenytoin and one group received daily intramuscular injection of phenytoin solution for 14 days. The 3rd group received no treatment following the operation. All the rabbits were euthanized 21 days post operation and samples were collected for histopathologic, electron microscopic and biomechanical investigations. There were no significant differences in wound closure time between the 3 groups and between the deep and superficial wounds. None of the biomechanical differences between the 3 groups and between the superficial and deep wounds in the same group were significant. The histopathological and ultrastructural results showed improvement in the architecture of the parenterally treated wounds compared to those treated topically. Concentration and the timing of the drug administration were considered to be the most important factors affecting the results. Lower doses seem to be more effective, although the reasons for this remain unclear.
Aboutorab Tabatabaei Naeini , Ahmad Oryan , Seifollah Dehghani and Behrooz Nikahval , 2008. Effects of Topical and Parenteral Application of Phenytoin on Cutaneous Wound Healing in Rabbits. Journal of Animal and Veterinary Advances, 7: 1537-1545.