Authors : Fernando Diosdado Vargas , Elvira Carrera Salas , Guadalupe Socci Escatel and Antonio Morilla Gonzalez
Abstract: The existence of sensitive and fast diagnostic techniques for the Porcine Reproductive and Respiratory Syndrome (PRRS) is very important because of the high presence frequency of the disease in farms causing problems in the swine industry. A commercial vaccine and organs from animals inoculated with the virus were used in order to establish a multiple Polymerase Chain Reaction (mPCR) technique. Total RNA extraction was done using Trizol® reactive. A simple RT-PCR assay established to amplify a 255 bp product from both, American and European strains. A nested multiplex method was standardized to obtain a 107 bp product from American strains and a 186 bp product from European strains. The desired 255 bp product was obtained form the positive controls as well as from other two mPCR products. The amplification of a 107 bp was achieved from serological and seminal samples, which pointed out that they corresponded to an American strain. It was possible to establish the mPCR technique is able to differentiate between American and European strains. Through out this technique, it will be possible to carry out epidemiological studies to determine the origin of PRRS virus infection, which will allow having a better control of the disease.
Fernando Diosdado Vargas , Elvira Carrera Salas , Guadalupe Socci Escatel and Antonio Morilla Gonzalez , 2008. Differentiation Between American and European Strains of the Porcine Reproductive and Respiratory Syndrome Virus in Mexico by the Establishment of a Multiple PCR. Journal of Animal and Veterinary Advances, 7: 17-20.