Authors : C.E. Larocca , I. Lago , M. Viqueira , R. Correa , W. Perez and J. Caravia
Abstract: The objective of the present research was to evaluate the effect of the addition of bovine Follicular Fluid (FF) to the In vitro Maturation media (IVM) of bovine oocytes. The Cumulus Oocyte Complexes (COC) were obtained by aspiration with PBS+bovine fetal serum (CS)+Antibiotics (ATB) from follicles measuring 2-5 mm in diameter from ovaries collected from a slaughter house. The FF was extracted from 15 mm follicles and was inactivated. In total, 718 COC (A and B) were selected and were randomly divided into two groups: G1 (Control, 329 COC), TCM+5% CS+ATB; G2 (FF, 389 COC), TCM-199/10% FF+ATB. Each group was then subdivided into 4 subgroups: [G1T1> 18h (69 COC); 20h (82 COC); G1T3: 22h (99 COC) and G1T4: 24 h (100 COC)]. COC were matured at 38.5�C, 5% CO2 and 99% humidity. The IVM of oocytes was evaluated by observing the first polar body using a stereoscopic comb (40X) and by visualizing the metaphasic plate of oocytes stained with 1% acetic orceine under the microscope (400X). The total number of mature COC for each group was: [G1T1: 18h (17), G1T2: 20h (28), G1T3: 22h (31) and G1T4: 24h (48); G2T1: 18h (38), G2T2: 20h (46), G2T3: 22h (47) and G2T4: 24h (49)]. Chi-square test was used with a significance level of 5%. These results show that the addition of FF enhaces the maturation percentages at 18, 20 and 22 h, with the best results obtained at 22 h. At 24 h percentages are similar between the 2 groups.
C.E. Larocca , I. Lago , M. Viqueira , R. Correa , W. Perez and J. Caravia , 2008. Effect of the Addition of Follicular Fluid on in vitro Maturation Media for Bovine Oocytes. Journal of Animal and Veterinary Advances, 7: 21-23.