Authors : Tonatiuh Alejandro Cruz Sanchez , Socorro Sandra Martinez Robles , German Garrido Farina , Jorge Tortora Perez , Susana Mendoza Elvira , Abel Ciprian Carrasco , Eliseo Hernandez Baumgharten , Andres Romero Rojas and Marco Antonio Vega-Lopez
Abstract: Mast cells (MC) and CD4+ and CD8+ cell subpopulations, from the apical border of the right cranial lobe of lungs of pigs experimentally infected with Mycoplasma hyopneumoniae, were histologically (MC) and immunohistochemically (CD4 and CD8) analyzed during the first 20 days post infection (PI). Twelve weaned, four-week-old healthy pigs were endotracheally inoculated with a lung homogenate infected with M. hyopneumoniae, strain 194 and 6 control animals were inoculated with Friis medium only. A control animal and two infected animals were euthanized at days 0, 4, 8, 12, 16 and 20 PI. M. hyopneumoniae induced an early increase of CD4 cells at day 4 PI (p<0.001) and at day 8 PI there was a significant and coincident increase in all three cell populations (p<0.001), which intermittently persisted until day 20 (CD8 p<0.001, MC p<0.001). The results of this work suggest that infection with M. hyopneumoniae induces an early response of CD4+ cells, followed by an infiltration of CD8+ cells and MCs, possibly related to the pathophysiological events of the disease, since, despite the evident immune infiltration, the infection continued its course.
Tonatiuh Alejandro Cruz Sanchez , Socorro Sandra Martinez Robles , German Garrido Farina , Jorge Tortora Perez , Susana Mendoza Elvira , Abel Ciprian Carrasco , Eliseo Hernandez Baumgharten , Andres Romero Rojas and Marco Antonio Vega-Lopez , 2008. In situ Analysis of CD4, CD8 and Mast Cells in Lung of Mycoplasma hyopneumoniae Experimentally Infected Pigs. Journal of Animal and Veterinary Advances, 7: 752-758.