Authors : Kamil Uney , Ayse Er , Gulcan Erbil Avci , Aziz Bulbul , Muammer Elmas and Enver Yazar
Abstract: The effect of tilmicosin on serum cytokine concentrations were investigated in healthy and lipopolysaccharide-treated mice. The mice were divided into 3 groups. Lipopolysaccharide (250 μg, Escherichia coli 0111:B4, intraperitoneally) was injected into the positive control group. The other 2 groups received tilmicosin (20 mg kg-1, subcutaneously) concurrently without or with lipopolysaccharide. After treatment, serum samples were collected at 0, 1.5, 3, 6, 12 and 24 h. Serum tumor necrosis factor, interleukin-1 and interleukin-10 levels were determined by enzyme-linked immunosorbent assay. Lipopolysaccharide increased all cytokine levels in the healthy mice. Tilmicosin slightly induced interleukin-1 production in the healthy mice, while it had no effect on tumor necrosis factor or interleukin-10 productions. However, tilmicosin elevated (p<0.05) tumor necrosis factor, interleukin-1 and interleukin-10 levels in lipopolysaccharide-treated mice. In conclusion, these data suggest that tilmicosin stimulates both proinflammatory and antiinflammatory cytokines at the dose recommended for infection.
Kamil Uney , Ayse Er , Gulcan Erbil Avci , Aziz Bulbul , Muammer Elmas and Enver Yazar , 2009. Effect of Tilmicosin on Serum Cytokine Levels in the Endotoxemia. Journal of Animal and Veterinary Advances, 8: 1021-1024.