Authors : Reza Jamshidi, Mohammad Mehdi Kasiriyan and Hasan Hafezeyan
Abstract: Phenotypic evaluation and culling of candidate animals for traits by applying traditional animal breeding are usually costly tasks, which require considerable time to be carried out. Molecular genetic as an alternative method, enables animal breeders to select eligible animals for the desirable trait (s) at their earlier ages. Selection based upon markers could result in increasing accuracy as well as selection response of animals. BMP15 gene is known as a candidate because it is responsible for high ovulation and multiple births in sheep. In this study, using for identification FecXI mutation in two exon of BMP15 gene, having taken blood samples of 150 sheep (140 ewes, 10 rams) from sheep in Damghan genetic modification center, using venojects treated with the anti-clot substance (EDTA), the DNAs were salted out and extracted. After the extraction and quantitative and qualitative tests (80% spectrophotometer and gel agarose 8%) the required amounts for each Polymerase Chain Reaction (PCR) were specified. After the polymerase chain reactions, using the relevant primer, the related part was reproduced (154) and then the PCR products were cut by XbaI enzymes for this gene, were two 30 and 124 bp is produced for target sites. In the sample with specified genotype in this study, only wild alleles (+) were observed and all samples had (++) genotypes.
Reza Jamshidi, Mohammad Mehdi Kasiriyan and Hasan Hafezeyan, 2009. 1Application of PCR-RFLP Technique to Determine BMP 15 Gene Polymorphism in Sangsari Sheep Breed of Iran. Journal of Animal and Veterinary Advances, 8: 1906-1910.