Authors : H.R. Bermudez, E.T. Renteria, B.G. Medina, S. Hori-Oshima, De La Mora VA Valle, V.G. Lopez, W.L. Yu, G.R. Pio, J.C. Herrera, C. Pujol and K. Nielsen
Abstract: About 553 Dairy cattle carcasses were inspected at a slaughter house in Baja California, out of 298 showed gross tuberculosis-like lesions. Microscopic examination of tissues using H and E and acid fast staining revealed 268 suspect cases. Of the 268, 104 were culture positive while 123 were PCR positive for M. tuberculosis complex. A total of 70 samples were culture and PCR positive and an additional 53 samples were PCR positives but culture negative. Thus PCR increases the rate of detection and will be a useful tool for control programs especially when considering the lower sensitivity of diagnosis by bacteriological culture, the occupational risk, technical difficulties with the protocol and the prolonged time of incubation.
H.R. Bermudez, E.T. Renteria, B.G. Medina, S. Hori-Oshima, De La Mora VA Valle, V.G. Lopez, W.L. Yu, G.R. Pio, J.C. Herrera, C. Pujol and K. Nielsen, 2010. Correlation Between Histophatological, Bacteriological and PCR Diagnosis of Bovine Tuberculosis. Journal of Animal and Veterinary Advances, 9: 2082-2084.