Authors : Wei Sun, Hong Chang, Kenji Tsunoda, Xinjun Liao, Zhangping Yang, Huaping Cheng and Da Li
Abstract: It was studied with Tan sheep, small-tailed Han sheep, Hu sheep, Tong sheep and Wadi sheep and their close related species goat was served as a referenced population. The distribution of gene frequency of 14 polymorphic structure loci and 7 microsatellite sites was detected, the result showed that: The variance analysis of the Heterozygosity (H), Polymorphic Information Content (PIC), effective allele Number (Ne) of alleles at each structural locus showed there were not significant differences either between species or among breeds (p>0.05). The variance analysis of the same 3 genetic indexes at microsatellite makers showed that there were not significant differences among populations in heterozygosity and PIC (p>0.05) as for effective allele number there were not significant differences both among Tan sheep, Hu sheep, Tong sheep and Wadi sheep, and between Wadi sheep and small-tailed Han sheep (p>0.05) but between the pre-four populations and the post-two populations there were significant difference (0.01>p>0.05). The variation levels of Tan sheep was the highest in 5 sheep populations followed by Tong sheep, Hu sheep, small-tailed Han sheep and then Wadi sheep. The variation level of small-tailed Han sheep was the highest in the 5 populations based on microsatellite makers followed by Wadi sheep, Tong sheep, Tan sheep and then Hu sheep. The results of population genetic equilibrium status based on the 2 level makers were different as to structure loci, Hu sheep, Tong sheep and Tan sheep were in the status of Hardy-Weinberg extreme disequilibrium (p<0.01) and Yangtse River Delta White goat, small-tailed sheep and Wadi sheep were in Hardy-Weinberg disequilibrium as to microsatellite makers, Hu sheep and goat were in the status of Hardy-Weinberg extreme disequilibrium (p<0.01) and other populations including Tong sheep, small-tailed Han sheep, Tan sheep and Wadi sheep were in Hardy-Weinberg disequilibrium (p<0.05). The average coefficient of gene differentiation (Gst) at each structural locus in 5 sheep and one goat populations was all higher than that in 5 sheep populations and the average coefficient of gene differentiation (Gst) at structural loci all was higher than that at microsatellite loci.
Wei Sun, Hong Chang, Kenji Tsunoda, Xinjun Liao, Zhangping Yang, Huaping Cheng and Da Li, 2010. Comparative Research on Genetic Diversity of Main Sheep Breeds of Mongolia Sheep Group in China Using Structure Loci and Microsatellite Makers. Journal of Animal and Veterinary Advances, 9: 2279-2283.