Authors : H. Furuta, T. Sanada, R. Takayanagi and T. Yoshida
Abstract: It was proved that an exogenous gene was successfully transferred to chicken embryo and Primordial Germ Cells (PGCs) by the injection of the blastoderm. In this experiment, an exogenous gene, Lac Z constructs encoding Escherichia coli beta-galactosidase was introduced into chicken blastoderm (stage X) just ovoposition using lipofection reagent. After injection of gene the eggs were incubated in routine manner until developmental stage 12-15. Survival rate of the treated embryo was 35.6%. Lac Z expression was mosaic manner and low efficiency. However, it was obtained Lac Z specific band from chicken PGCs by PCR method.
H. Furuta, T. Sanada, R. Takayanagi and T. Yoshida, 2010. Introduction of Exogenous Gene into Chicken PGCs via Blastoderm. Journal of Animal and Veterinary Advances, 9: 748-750.