Authors : Xiaoling Ding, Yueyun Ding, Chenghua Zhang, Long Huang, Xiaolei He and Zongjun Yin
Abstract: In order to give a basis for molecular breeding of meet quality traits in Wannan black pig, we applied PCR-RFLP method to analyze the polymorphism of 5'upstream region and intron 2 of H-FABP gene in 184 Wannan black pigs population. The results show that there exist polymorphic loci of restriction enzyme Hinf in 5'upstream region and Hae III as well as Hinf I in intron 2. Except for locus of restriction enzyme HinfI, the other two polymorphism sites achieved in Hardy-Weinberg equilibrium based on χ2-test (p>0.05). In addition, a T→C mutation in 1324 bp, a C→G mutation in 1811 bp and a C→T mutation in 1970 bp were found through the direct sequencing the polymorphic RFLP fragments of H-FABP gene, then genotype frequencies, gene frequencies, Ho, He, Ne and PIC were investigated in Wannan black pig population. The results identified 3 variant restriction sites and provided a basic data for further study of the relationship between IMF and H-FABP with different genotypes and also provide basic for make sure whether H-FABP gene is the key gene for effect on the deposition of IMF.
Xiaoling Ding, Yueyun Ding, Chenghua Zhang, Long Huang, Xiaolei He and Zongjun Yin, 2011. Polymorphisms and Sequencing Analysis in 5'Upstream and Intron 2 Region of H-FABP Gene in Anhui Wannan Black Pig Population. Journal of Animal and Veterinary Advances, 10: 1568-1572.