Authors : V. Vega-Sanchez, C. Salgado-Miranda, S. Lagunas-Bernabe, N.L. Calderon-Apodaca and Edgardo Soriano-Vargas
Abstract: The ability of nine reference strains of Ornithobacterium rhinotracheale (serovars A-I) to raise detectable, hemagglutination-inhibition antibodies in immunized specific-pathogen-free chickens was investigated. Six of the nine O. rhinotracheale serovar (A-H) reference strains raised detectable hemagglutination-inhibition antibodies 14 and 21 days after vaccination. Detectable serum hemagglutination-inhibition antibody titers ranged from 512-16348. Furthermore, transmission electron microscopic examination of a hemagglutinating reference strain (B 3263/91, serovar A) demonstrated a close interaction between the bacterial and chicken erythrocyte surfaces. The results indicate that most of the hemagglutinating O. rhinotracheale reference strains included in this study are able to raised detectable hemagglutination-inhibition antibodies in immunized specific-pathogen-free chickens. The findings have the potential to become an alternative laboratory approach for detecting serum antibodies from chickens immunized with O. rhinotracheale.
V. Vega-Sanchez, C. Salgado-Miranda, S. Lagunas-Bernabe, N.L. Calderon-Apodaca and Edgardo Soriano-Vargas, 2011. Reference Strains of Ornithobacterium rhinotracheale Raise Detectable Hemagglutination-Inhibition Antibodies in Immunized Specific-Pathogen-Free Chickens. Journal of Animal and Veterinary Advances, 10: 1623-1626.