Authors : Tulay Ifakat Cagatay and Jon Hickford
Abstract: In this study, we reported rapid approach for identification of D. nodosus serotypes that causes footrot, higly contagious diseases of the ruminants in worldwide with using Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) analysis. A total of 500 swab samples from infected hoof lesion were investigated for presence of D. nodosus by PCR amplification of fimA gene. PCR-SSCP patterns revealed that each sample contained two or three different D. nodosus serotypes. Additionally on the of basis SSCP patterns, 8 different serogroups (A- G and I) were identified from New Zealand samples. These findings were confirmed by cloning and sequences of fimA gene.
Tulay Ifakat Cagatay and Jon Hickford, 2011. Serotyping Dichelobacter nodosus with PCR-SSCP. Journal of Animal and Veterinary Advances, 10: 1678-1682.