Authors : Li Yu, Fang Yan-Hong, Zhu Heng-Wen, Sun Pei, Wei Jian-Zhong and Yin Zhong-Jun
Abstract: To investigate molecular epidemiological characteristic of Salmonella isolated strains in pig carcass, the current study, 71 strains of Salmonella genome DNA were isolated from the muscle and surface of pig carcass samples in five designated slaughterhouses in Hefei, Anhui province, China. They were gathered and analyzed using the technique of Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR). The multiple DNA fragments of all strains generated with ERIC2 primers were composed of 6-14 bands ranging in sizes between 250 bp and 3 kb. One characteristic band (approximately 250 bp) was conserved among nearly all isolates. This study showed that genotype polymorphism exists in Salmonella isolated strains, serotype and the source of the isolated strains with the same genotype had certain relevance. It further proves that the carcass surface contamination during slaughter is mainly from cross-contamination and environmental pollution during product processing and carcass meat-like isolates were from the endogenous contamination.
Li Yu, Fang Yan-Hong, Zhu Heng-Wen, Sun Pei, Wei Jian-Zhong and Yin Zhong-Jun, 2011. The Analysis of ERIC-PCR Genomic Polymorphism of Salmonella Isolated Strains in Pig Carcass. Journal of Animal and Veterinary Advances, 10: 1694-1698.