Authors : Zhihua Cai, Like Wang, Deyi Liu, Shenghe Li, Xunsheng Pang and Yuxin Cheng
Abstract: In this study, IRF3 gene was chosen as a candidate gene for evaluating its effect on porcine immune traits. A SNP (HQ026024: g.4252T>C) in exon7 of IRF3 gene was demonstrated by PCR-SSCP analysis and sequencing. Immune traits including IFN-γ and IL10 concentrations in serum were measured when the pigs are at 20th day of age. The further association analysis of SNP genotypes with immune traits were also conducted in 3 pig breeds including Large white, Landraces and Chinese indigenous breed Dingyuan black pig. The results indicated that the SNP of IRF3 gene had highly significant effects on level of IFN-γ and IL10 (day 20) in serum (p = 0.0344; p = 0.0121) and ratio of IFN-γ to IL10 (day 20) in serum (p = 0.0315). The results suggested that the IRF3 gene could be regarded as a molecular marker gene for genetic selection of these immune traits in the pig breeding program.
Zhihua Cai, Like Wang, Deyi Liu, Shenghe Li, Xunsheng Pang and Yuxin Cheng, 2011. Polymorphism Identification and Association Analysis of IRF3 Gene in Pig. Journal of Animal and Veterinary Advances, 10: 1841-1844.