Authors : J.Y. Bai, W. Li, S. Li, W.P. Zhang, C.Q. Gu, X.Y. Hu and G.F. Cheng
Abstract: To study the cross-application of the Antibodies (Abs) of Homo sapiens and duck origins which provides the fundamentals for potential application of related Abs, we analyzed their differences by molecular biology techniques and preliminary application in peripheral blood. The similarities of deduced aa sequences of CD8α gene reached to about 32% between cherry valley duck and homo sapiens whereas the extracellular regions reached to about 30%. The result showed the obvious differences in protein hydrophilicity, antigenicity and the possibilities of CD8α extracellular region. This study successfully expressed the extracellular region of cherry valley ducks CD8α (ERCVCD8) sequence and prepared for corresponding Abs. The percentage of control group (group 1) CD8+lymphocytes was relatively stable during different phases of the post-infected with DPV when using rabbit anti-the recombinant protein of extracellular region of cherry valley ducks CD8α (rCVERCD8) serum but the percentage of CD8+lymphocytes was unstable when using anti-Homo sapiens CD8α mAb. When rabbit anti-rCVERCD8 serum was used, the percentage of infection group (group 2) CD8+lymphocytes showed regularity however that of CD8+lymphocytes using rabbit anti-Homo sapiens CD8α Abs was irregular. This study indicats that the identification of cherry valley ducks CD8+lymphocytes using anti-Homo sapiens CD8α Abs has its limitations.
J.Y. Bai, W. Li, S. Li, W.P. Zhang, C.Q. Gu, X.Y. Hu and G.F. Cheng, 2011. The Identification of Cherry Valley Ducks CD8+Lymphocytes by Using Homo sapiens CD8 Antibodies. Journal of Animal and Veterinary Advances, 10: 2516-2522.