Authors : Jiang Caode and Liu Yonggang
Abstract: The full-length cDNA sequences of one sheep gene, SERPINF1 was amplified using the Rapid Amplification of cDNA Ends (RACE) method based on one pig EST sequence which was highly homologous to the coding sequence of human SERPINF1 gene. Sequence prediction analysis revealed that the open reading frame of this gene encodes a protein of 416 amino acids that has high homology with the serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 (SERPINF1) of twelve species-bovine (96%), pig (91%), human (88%), chimpanzee (88%), horse (88%), crab-eating macaque (87%), dog (89%), domestic guinea pig (84%), mouse (85%), rat (83%), red jungle fowl (63%) and western clawed frog (55%) so that it can be defined as sheep SERPINF1 gene. This novel sheep gene was assigned to GeneID: 100192425. The phylogenetic analysis revealed that the sheep SERPINF1 gene has a closer genetic relationship with the SERPINF1 gene of bovine. Tissue expression analysis indicated that the sheep SERPINF1 gene is differentially expressed in detected tissues including spleen, muscle, skin, kidney, lung, liver, fat and heart. The experiment is the first to establish the primary foundation for further research on the sheep SERPINF1 gene.
Jiang Caode and Liu Yonggang, 2011. Isolation, Sequence Identification and Tissue Expressio Profile of a Novel Sheep Gene-SERPINF1. Journal of Animal and Veterinary Advances, 10: 2593-2598.