Authors : Jiang Caode, Liu Yonggang and Xia Xueshan
Abstract: The complete coding sequences of three sheep genes-RAB1A, RAB4A and RAB5A were amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Sequence analysis revealed that the sheep RAB1A gene encodes a protein of 204 amino acids that shares high homology with the RAB1A, member RAS oncogene family (RAB1A) proteins of nine species-human (98%), mouse (98%), rat (98%), pig (98%), zebrafish (98%), Western clawed frog (97%), Atlantic salmon (96%), disc abalone (92%) and great pond snail (87%). The sheep RAB4A gene encodes a protein of 218 amino acids that shares high homology with the RAB4A, member RAS oncogene family (RAB4A) proteins of eight species-cattle (100%), dog (99%), human (99%), rhesus monkey (99%), horse (99%), chicken (99%), rat (98%) and mouse (98%). The sheep RAB5A gene encodes a protein of 215 amino acids that shares high homology with the RAB5A, member RAS oncogene family (RAB5A) proteins of fifteen species-cattle (98%), rabbit (98%), dog (98%), horse (98%), pig (98%), sumatran orangutan (98%), human (97%), mouse (97%), rat (97%), chimpanzee (97%), chicken (95%), rhesus monkey (93%), African clawed frog (93%), Western clawed frog (93%) and zebrafish (92%). Finally, these three novel sheep genes were assigned to GeneIDs: 100302065, 100302066 and 100302084. Phylogenetic analysis indicated that the sheep RAB1A gene has a closer genetic relationship with the RAB1A genes of human, mouse and rat. The sheep RAB4A and RAB5A genes both have closer genetic relationships with the RAB4A and RAB5A genes of cattle. Tissue expression profile analysis was also carried out and results demonstrated that sheep RAB1A, RAB4A and RAB5A genes were all generally but differentially expressed in detected tissues.
Jiang Caode, Liu Yonggang and Xia Xueshan, 2011. Molecular Cloning, Sequence Identification and Tissue Expression rofile of Three Novel Sheep (Ovis aries) Genes-RAB1A, RAB4A and RAB5A. Journal of Animal and Veterinary Advances, 10: 2659-2665.