Authors : Fatemeh Farahi, Nima Khoramabadi, Esmaeil Asli, Ashraf Mohabati Mobarez and Abdolaziz Rastegarlari
Abstract: Available vaccines against Brucella species are live attenuated Brucella strains with some disadvantages. So as to design an improved vaccine to be used in animals and humans, the researchers aim to engineer a subunit brucellosis vaccine. Immunologic evaluation of Outer Membrane Proteins (OMPs) of Brucella cells has a key role in development of prevention and diagnosis programs. Particularly, the researchers are interested in the 18 kDa outer membrane lipoprotein of Brucella abortus (Omp19) as it is most exposed to the host immune system and is anticipated to be capable of eliciting immune response. As the immunodominant antigen of smooth Brucellae is LPS, the principle agent of serodiagnosis of the infection, the researchers used detoxified Lps which loses fatty acid chain and includes compelet polysacharid fragment. The aim of this study is to produce a serological assessment of recombinant Omp19 (rOmp19) cojugated with detoxified Lps in a way to prevent brucellosis. Brucella omp19 gene was cloned in pET28a (+) and expressed in E. coli BL21 (DE3) by 1 mM IPTG. Recombinant protein was purified using nickel resin and confirmed by western blot analysis using polyclonal antibodies and conjugated with detoxified Lps which was applied to detect antibodies in BALB/c mice brucellosis cases’ serum samples via western-blotting. Overall these results indicate that rOmp19 conjugated with detoxified Lps would be a useful candidate for a subunit vaccine against human and animal brucellosis.
Fatemeh Farahi, Nima Khoramabadi, Esmaeil Asli, Ashraf Mohabati Mobarez and Abdolaziz Rastegarlari, 2011. Immunologic Evaluation of Surface 18 kDa Protein of Brucella abortus S19 Cojugated with Detoxified Lps as an Antigen Useful for Human Brucellosis Vaccine in BALB/c Mice Model. Journal of Animal and Veterinary Advances, 10: 3085-3089.