Authors : Binru Shang, Yiqiang Chen, Zongyi Wang, Wenjun Yang and Liying Zhang
Abstract: A new method for simultaneous determination of melamine and cyromazine in animal feeds using Gas Chromatography-Mass Spectrometry (GC-MS) was developed and validated. Samples were extracted with trichloroacetic acid solution cleaned up by cation exchange solid-phase extraction cartridges and derivatized with N, O-bis (trimethylsilyl) trifluroacetamide containing 1% trimethylchlorosilane followed by GC separation and MS detection. The limits of quantification were 0.10 mg kg-1 for both melamine and cyromazine. Recoveries from feeds spiked at levels between 0.1 and 50 mg kg-1 ranged from 84.2-99.5% with Relative Standard Deviation (RSD) <8% with the exception of a 10.2% RSD for 0.1 mg kg-1 melamine. This validated method was successfully applied to commercial feed samples showing that it can be used as a routine tool for the surveillance and evaluation of the presence of melamine and cyromazine in animal feeds.
Binru Shang, Yiqiang Chen, Zongyi Wang, Wenjun Yang and Liying Zhang, 2011. Development and Validation of a Gas Chromatography-Mass Spectrometry Method for the Simultaneous Determination of Melamine and Cyromazine in Animal Feeds. Journal of Animal and Veterinary Advances, 10: 73-80.