Authors : Qi Renli, Sun Chao , Yan Jun , Yang Haili , Zhao Taotao and Zhao Xia
Abstract: The present study was carried out to illustrate the effect of glucose on chicken (Gallus gallus) preadipocyte differentiation and fat metabolism. Adipocyte differentiation was initiated by maintaining confluent cells in a glucose-free medium supplemented with different concentrations of glucose. Upon exposure to high concentrations of glucose (25 mmol L-1), Peroxisome Proliferator-Activated Receptor γ (PPARγ) and CCAAT/Enhancer-Binding Protein α (C/EBPα) as adipocyte differentiation markers were significantly increased compared with control cells. The morphology of the glucose-treated cells changed from fibroblast-like to polygonal and cells treated with moderate concentrations of glucose (15 mmol L-1) accumulated the most number of cytoplasmic lipid droplets as estimated by Oil red O staining. Compared with control cells varying concentrations of glucose affected the mRNA expression and protein levels for Fatty Acid Synthesis (FAS) and Adipose Triglyceride Lipase (ATGL), the master regulators of fat metabolism. Moreover, the mRNA expression levels and protein contents of fatty acid transporters in glucose-treated cells were higher than in untreated cells. These results indicated that glucose is essential material for chick adipocytes differentiation. Moreover, overhigh concentrations of glucose maybe stimulate lipolysis in the cells.
Qi Renli, Sun Chao , Yan Jun , Yang Haili , Zhao Taotao and Zhao Xia , 2012. Effects of Glucose on Differentiation and Fat Metabolism of Chicken Preadipocytes. Journal of Animal and Veterinary Advances, 11: 1223-1229.