Authors : Guang-Ping Ruan, Jin-Xiang Wang , Rong-Qing Pang , Xiang Yao , Yong-Li Deng , Qiang Wang , Li-Hua Ma and Xing-Hua Pan
Abstract: The liver cells of fluorescent male mice were transfused to half-lethally irradiated C57BL female mice. Whether liver cells had hematopoietic reconstruction function was investigated. Researchers used liver cells of fluorescent male mice to transfuse back to half-lethally irradiated C57BL females mice. At 18, 39, 53 days after transplantation, mice were cut tails to collect blood and with CD4-PE and CD8-PE labeled and then bloods were hemolyzed by ammonium chloride. Then, the cells were detected by flow cytometry. About 87 days after transplantation, mice were killed to collect bone marrow and detected Y chromosome positive percentage by fluorescence in situ hybridization. GFP positive cells were detected in peripheral bloods, spleen cells, liver cells and bone marrow cells of three sacrificed mice. The female mice infused with liver cells were detected GFP-positive cells in peripheral blood and some GFP-positive cells converted into CD4+ or CD8+T cells. The percentage of GFP-positive cells in blood was positive correlated to the proportion of Y chromosome in bone marrow detected by fluorescence in situ hybridization analysis. GFP positive cells can be detected in peripheral bloods, spleen cells, liver cells and bone marrow cells of three sacrificed mice. The percentages of GFP-positive cells detected in the spleen cells were higher than other cells with statistical significance (p = 0.003). The adult mice liver cells contained a certain amount of hematopoietic stem cells. These cells survived in female mice and distributed in various organs and could reconstruct hematopoietic function.
Guang-Ping Ruan, Jin-Xiang Wang , Rong-Qing Pang , Xiang Yao , Yong-Li Deng , Qiang Wang , Li-Hua Ma and Xing-Hua Pan , 2012. Liver Cells of GFP Transgenic Male Mice Transplantation to Reconstruct Hematopoietic Function in Female Mice. Journal of Animal and Veterinary Advances, 11: 1258-1264.