Authors : Liang ZiWen, Wu QiNan, Tang QiuShan, Chen Bing, Wu Ping, Gao Yuan and Xiao Qian
Abstract: To investigate the over expression of PPARα and trimetazidine in the apoptosis of rat cardiomyocyte with type 2 diabetes. All rats were divided into 3 groups: Group N (control group), Group D (type 2 diabetes group); Group T (type 2 diabetes which treated by trimetazidine). After 16 weeks breeding, apoptosis of each group were detected by TUNEL, ultrastructure were detected by electronic microscope, immunohistochemistry staining and western blotting were used to detected each group’s cardiomyocyte about PPARα and NF-κB in the expression of each protein level. The number of apoptosis was increased in Group D and the ultra structure was changed, the expression of PPARα in protein level was decreased also the NF-κB (p<0.05). After treated by trimetazidine in type 2 diabetic rat, the apoptosis of cardiomyocytes was decreased distinctly (p<0.05), the change of ultra structure was improved, the expression of PPARα and NF-κB were all decreased in protein level (p<0.05). The over expression of PPARα might be involved in the regulation of the apoptosis in cardiomyocyte and the ultrastructual change of diabetic cardiomyopathy, suggesting it may participate in the development of diabetic cadiomyopathy and treated by trimetazidine may reverse it.
Liang ZiWen, Wu QiNan, Tang QiuShan, Chen Bing, Wu Ping, Gao Yuan and Xiao Qian, 2012. Effect of PPARα’s over Expression on the Type 2 Diabetic Rat Cardiomyocytes. Journal of Animal and Veterinary Advances, 11: 1588-1594.