Authors : Junlin Zhang, Yulei Wei, Mingming Qin, Fan Yang and Huayan Wang
Abstract: CAMA-syn, a hybrid composed of N-terminal α-helical segment of CA (amino acid 1-8) and N-terminal α-helical segment of MA (amino acid 1-12) is a novel small peptide with the potent antibacterial and synergistic activity without cytotoxicity. In order to test the antibacterial function of CAMA-syn produced specially in lung epitheliuml cells, several vectors containing the synthesized CAMA-syn DNA fragment initiated by CMV and Surfactant Protein C (SP-C) promoter were constructed and transfected into recipient cells. The results showed that CAMA-syn fusion to Green Fluorescent Protein (GFP) or to Hemagglutinin epitope (HA) tag was expressed in both 293 and A549 cells. The antibacterial assays of CAMA-syn were conducted against both Gram positive and negative bacteria including S. abortusovis, P. anatis, S. hyicus and S. suis. The results of colony forming efficiency and cell growth curves proved that the in vitro expressed CAMA-syn could inhibit the growth of bacterial, demonstrating that lung epithelial cell-specific expression of antimicrobial peptide CAMA-syn could have antibacterial activity.
Junlin Zhang, Yulei Wei, Mingming Qin, Fan Yang and Huayan Wang, 2012. Transgenic Expression and Antimicrobial Potential of CAMA-Syn in A549 Cells. Journal of Animal and Veterinary Advances, 11: 2006-2012.