Authors : Zhong Fei, Wang Yin, Li Li-Rui, Huang Hai-Yan, Song Yong, Yang Ze-Xiao and Yao Xue-Ping
Abstract: To provide the evidence for detection and epidemical investigation of Haemophilus parasuis by means of isolation and identification of Haemophilus parasuis and study on its detection method of ELISA. From the pathological specimens collected from several scaled-up pig farms, the strain of Haemophilus parasuis was isolated identified by means of observation of cultural characteristics and morphology and structure, Biochemical tests, Antibiotic Susceptibility tests, PCR identification and serovar identification. Subsequently, indirect ELISA Method of Haemophilus parasuis antibody for detection was studied by utilizing the isolated strain bacteria of treated with ultrasonication as the antigen with preparation of positive serum, optimization of concentration of the coating antigen, concentration of the secondary antibody and reaction time and Susceptibility test, Specificity test and Repeatability test. A strain of Haemophilus parasuis serovar 5, named as SCHPS5 which is sensitive to cephalosporin IV, cefotaxime, sulbactam, furaxone, chloromycetin. The detection sensitivity of the target antibody in indirect ELISA Method based on this isolate can reach over 1:10000 and this detection method has high specificity and high repeatability. This study has provided scientific evidence and a reliable antibody detection method for prevention and treatment of the disease resulting from Haemophilus parasuis.
Zhong Fei, Wang Yin, Li Li-Rui, Huang Hai-Yan, Song Yong, Yang Ze-Xiao and Yao Xue-Ping, 2012. Isolation and Identification of Haemophilus parasuis and Establishment of its Detection Method of Indirect ELISA. Journal of Animal and Veterinary Advances, 11: 2384-2391.