Authors : Heng Lei, Huili Wang, Tingting Ning, Wei Hao and Chuncheng Xu
Abstract: This study was to select strains which have a strong ability to degrade starch and strain which has a strong ability to synthesize protein from nine strains of three species for mixed strains fermentation of potato starch residue to enrich the protein content for further use as an animal feed and to investigate the optimal combination of selected strains and technical parameters of fermentation process. In the experiment of single strain fermentation, three strains, Candida tropicalis AS 13, Saccharomycopsis fibuligera JS2 and Geotrichum candidum AS 2.2342 were selected for mixed strains fermentation by comparison the degradation rate of starch and the synthesis rate of protein. In the experiment of mixed strains fermentation, the True Protein (TP) content were obtained in the substrate fermented by the three strain combination was higher than two-strain combinations. Then the effects of technical parameters such as inoculation ratio, inoculation method and total inoculum size of solid state fermentation on the TP content were analyzed, respectively. The experiment results showed that the use of potato starch residue fermented with the three-strains combination of C. tropicalis AS 13, S. fibuligera JS2 and G. candidum AS 2.2342 at the ratio of 1:7:2 with total 10 mL inoculum size at the same time can improve TP content by up to 16.1% which would subsequently provide a good feedstuff for ruminants.
Heng Lei, Huili Wang, Tingting Ning, Wei Hao and Chuncheng Xu, 2012. Protein Enrichment of Potato Starch Residue by Solid State Fermentation with Mixed Strains. Journal of Animal and Veterinary Advances, 11: 2700-2705.