Authors : Fugui Fang, Xiuhong Zhou, Fubao Li, Zhizhong Zhang and Shudong Jiang
Abstract: Glucagon is the hyperglycemic factor that opposes the action of insulin. It stimulates the breakdown of liver glycogen and increased glucogeogenesis. Glucagon is located in the tissues of the pancreas of many animals. However, the distribution of glucagon has not been investigated in the pancreas of the tiger. The present study was performed to describe the histological structure of the liver and pancreas and the location of glucagon of pancreas in the 8 days old Siberian tiger. The tissues of the liver and pancreas were collected from three 8 days old Siberian tigers that died of starvation. The weight was recorded and the histological sections were made and stained with HE. The localization of glucagon in tissue was detected by immunohistochemical two-step PV-9000. The results show that the average weight of the liver and pancreas was approximately 39.06 and 1.97 g, respectively. The tissue structure of the liver consisted of hepatic lobulation and portal area. Central vein, hepatocyte tube and hepatic sinusoid and lymphatic tissue were found in hepatic lobulation. Interlobular veins, interlobular artery and interlobular bile duct were presented in portal area. The pancreas was basically made up of exocrine portion and pancreas islet. The exocrine secretion of the pancreas included many pancreatic acinuses and centroacinar cell was observed in the pancreatic acinus. Glucagon-immunopositive cells were mainly located in the pancreas islet. The current study preliminarily revealed the histological features of the liver and pancreas and the distribution of glucagon in the pancreas of 8 days old Siberian tiger which requires further investigation.
Fugui Fang, Xiuhong Zhou, Fubao Li, Zhizhong Zhang and Shudong Jiang, 2012. The Histological Structure of the Liver and Pancreas and Distribution of Glucagon of Pancreas in the Siberian Tiger (Panthera tigris altaica). Journal of Animal and Veterinary Advances, 11: 2885-2889.