Abstract: The experiment was conducted to evaluate the effects of N-Carbamylglutamate (NCG) on intestinal mucosa with its proliferation in weanling piglets. About 12 Duroc x Landrace x Yorkshire piglets (average BW 5.67±0.14 kg; weaned at 21 days) were grouped into 2 treatments and fed one of the following diets for 7 days: a Standard Diet (SD), SD+NCG (0.05%). All the piglets were sacrificed for blood and tissue collection. The results showed that compared with the control group, NCG decreased serum urea nitrogen and ammonia concentration (p<0.05). Adding NCG to the diet resulted in a higher villus height in jejunum (p<0.05). The RT-PCR results showed that NCG significantly increased PCNA mRNA abundance in both jejunum (p<0.05) and ileum (p<0.01). These results indicated that oral supply of NCG improved intestinal mucosa morphology and had favorable effects on proliferation of intestinal mucosa.