Authors : Wan Hongping, Zuo Zhicai, Fu Tongchao, Dai Hongbo, Chen Lei, Xu Zhiwen and Zhu Lin
Abstract: A highly infectious and fatal respiratory tract disease occurred in a swine farm in Sichuan province. To explore the reason of the disease, samples of the liver and lung tissue, acquired sterilely in a typically sickened pig were intended for pathogenic detection. The suspicious strain, named after SCAB-1 was diagnosed which depended on cultural characteristics, morphologic observation and Nicotinamide Adenine Dinucleotide (NAD) experiment. Its pathogenicity was diagnosed by the virulence test on mice. And the strain was exactly identified by the analysis of 16S rDNA sequence with its resistance inspected by sensitive test. According to cultural characteristics, morphology, NAD experiment and the analysis of 16S rDNA sequence, it revealed that the study triumphantly isolated a strain of Actinobacillus pleuropneumonia which could lead a mouse to death, after infected 16 h and depend on NAD. The homology of the 16S rDNA sequence between SCAB-1 and Actinobacillus pleuropneumonia is highly 99.9%. SCAB-1, Actinobacillus pleuropneumonia is susceptible to ceftriaxone, cefotaxime, cefuroxime, norfloxacin, SMZco; moderately sensitive to cefazolin, cefoperazone and resistance to cephaloridine IV, vancomycin and gentamycin.
Wan Hongping, Zuo Zhicai, Fu Tongchao, Dai Hongbo, Chen Lei, Xu Zhiwen and Zhu Lin, 2012. Isolation and the Analysis of 16S rDNA Sequence of Swine Actinobacillus pleuropneumonia. Journal of Animal and Veterinary Advances, 11: 3276-3280.