Authors : Min Dai, Cheng Peng, Feng Wan and Fu Peng
Abstract: Minimum Inhibitory Concentrations (MICs) of 3 extracts and oil from Pogostemon cablin against isolates from milk produced by dairy cow with mastitis were determined by Agar Dilution Method. Water extract had low activity; ethanol extract and ethanol-water extract had moderate effects having MICs ranging from 25.0-100.0, 12.5-100.0 mg mL-1, respectively. Oil had strong activity against Gram-positive and Gram-negative isolates with MICs ranging from 0.2-8.2 mg mL-1. Oil also, showed strong efficacy in mouse peritonitis model infected with S. aureus or E. coli. Their actions were dose-dependent. Obvious morphological changes were observed by TEM in oil-treated E. coli or S. aureus such as a remarkable electron-light region small electron-dense granules disturbed cytoplasmic membrane and abnormal cell division. In conclusion, antibacterial substance of P. cablin was oil which had broad-spectrum antibacterial activities so to make it a potential candidate as antibacterial agent to treat bovine mastitis.
Min Dai, Cheng Peng, Feng Wan and Fu Peng, 2012. Antibacterial Activity and Mechanism of Pogostemon cablin Against Bacteria from Milk of Dairy Cows Suffering with Mastitis. Journal of Animal and Veterinary Advances, 11: 3289-3297.