Authors : Xiangbo Xu and Bin He
Abstract: Vascular Endothelial Growth Factor (VEGF)/Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) are vital in menstruation. This study aimed to explore their expressions in a mouse menstrual-like model by pharmacologic progesterone withdrawal. A mouse model was prepared by administration of mifepristone after decidualization of ovariectomized mice. Semiquantive-PCR and in situ hybridization were performed to the expression patterns of VEGF/VEGFR-2 mRNA. VEGF mRNA peaked at 24 h and obviously reduced at other time points. VEGF mRNA was mainly located in the basal zone from 0-24 h, epithelium and stroma zone from 32-48 h. VEGFR-2 mRNA was higher at 0 and 40 h rather weak at 8, 16 and 32 h, nearly not expressed at 24 and 40 h. VEGFR-2 mRNA was strongly located in the basal zone at 0, 8 and 16 h in the epithelium zone at 24, 32 and 48 h and epithelium and stroma zone at 40 h. The locations of VEGF/VEGFR-2 in mouse menstrual-like model displayed regional character.
Xiangbo Xu and Bin He, 2012. VEGF/VEGFR-2 mRNA Expression in a Mouse Menstrual-Like Model by Pharmacologic Progesterone Withdrawal. Journal of Animal and Veterinary Advances, 11: 3512-3516.