Authors : Maria Antonia Coba Ayala, Guadalupe Socci Escatel, Laura Elena Zapata Salinas, Maria Elvira Carrera Salas and Enrique Chavez Castaneda
Abstract: The objective of this study was to establish the optimal conditions for carrying out the PCR technique to detect the Aujeszky’s Disease (AD) virus in tissues. For this purpose, researchers used different rabbit and pig tissue inoculated with the Shope strain of the AD virus as well as uninoculated animal tissues. Cell culture of the same strain was used as positive control. A commercial kit was employed for DNA extraction. DNA amplification was performed with a pair of primers flanking a 334 bp fragment of the gB (gII) gene. It was possible to obtain the expected product in 14 of 16 samples of tissues from infected animals. These results were consistent with those obtained by virus isolation. The assay sensitivity showed that the PCR can amplify DNA up to 65 fg. The specificity of the test was confirmed by failure to observe any amplification product from the 16 samples neither from negative animals nor from bovine herpesvirus DNA. It is a molecular method that may be useful to complement the diagnosis of the disease in Mexico.
Maria Antonia Coba Ayala, Guadalupe Socci Escatel, Laura Elena Zapata Salinas, Maria Elvira Carrera Salas and Enrique Chavez Castaneda, 2012. Polymerase Chain Reaction for Diagnosis of Aujeszky Disease in Mexico. Journal of Animal and Veterinary Advances, 11: 4217-4220.