Authors : Ayumi Suzuki, Naohiko Okumura, Yoshifumi Uehara, Hirohide Uenishi and Takashi Awata
Abstract: In this study, researchers developed a DNA-based identification method for the Tokyo X strain which is a pig brand developed by the Tokyo Metropolitan Livestock Experiment Station. To formulate the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Method, Single Nucleotide Polymorphisms (SNPs) were screened and a total of 104 SNP loci were fixed in the original Tokyo X strain of these, researchers selected 8 SNPs which are recognisable by restriction endonucleases and have high polymorphism within other major commercial breeds and market pork. The combined exclusion probability of general commercial pork and Berkshire pork from Tokyo X was 98.1 and 97.4%, respectively. The SNP marker set developed in this study will be useful in preventing fake Tokyo X brand pork from appearing on the market.
Ayumi Suzuki, Naohiko Okumura, Yoshifumi Uehara, Hirohide Uenishi and Takashi Awata, 2012. An Identification Method for the Tokyo X Strain; The Pig Brand of the Tokyo Metropolitan Agriculture and Forestry Research Center. Journal of Animal and Veterinary Advances, 11: 4236-4245.