Authors : Ai-Xin Liang, Fang-Xiao Dong, Xuan Luo, Xue Yu, Li Han, Yan-Guo Han, Ai-Zhen Guo and Li-Guo Yang
Abstract: In order to estimate the optimal immunization procedures for a novel somatostatin vaccine carried by Δcrp Δasd double deleted attenuated Salmonella enterica serovar Typhimurium. Mice were vaccinated with somatostatin vaccine strain (C500/pVGS/2SS-asd) by using three immunization dosages and three alternative schedules. The specific antibody response was evaluated by indirect ELISA Method. The results showed that 0.2x1010 CFU per mouse was the optimal immunization dosage to induce specific IgG response. Interestingly, the booster groups either 2 weeks interval or 4 weeks interval after the first immunization were not improving the level of specific IgG response as expected compared with only injection one dose groups. Furthermore, the different interval period of booster injections also affected the immune response, antibody levels of booster given 4 weeks interval was much higher than that booster given 2 weeks interval. Similar results were obtained on body weight gains analysis. The results of body weight gains of immunized mice also showed that the most effective immunization dosage is 0.2x1010 CFU per mouse and the optimal immunization number is a single dose. These observations have a very important application in the field of animal production.
Ai-Xin Liang, Fang-Xiao Dong, Xuan Luo, Xue Yu, Li Han, Yan-Guo Han, Ai-Zhen Guo and Li-Guo Yang, 2012. Immunization Procedures of Δcrp Δasd Double Deleted Attenuated Salmonella Enterica Serovar Typhimurium delivering Somatostatin Antigen in Mice. Journal of Animal and Veterinary Advances, 11: 4526-4532.