Authors : Yanying Zhang, Qiumei Shi, Guisheng Gao, Hai Fang, Cuizhen Chen, Gaili Ren, Huoju Chang and Donglin Zhang
Abstract: The detections of the Locus of Enterocyte Effacement Pathogenicity Island (LEE) and High Pathogenicity island (HPI) in 26 E. coli samples from chickens were conducted by PCR. Ler and eaeA genes located in the core of LEE Virulence Island while irp2, fyuA and asn_RNA_intB genes in HPI Pathogenicity Island were all detected. At the same time, O serotype identification of 26 E. coli samples were conducted. The results showed that the positive rate of Ler gene in LEE Virulence Island was 3.85% (1/26) while eaeA was 3.85% (1/26). The rate of fyuA gene in HPI pathogenicity island was 42.3% (11/26) while irp2 was 73% (19/26), irp2+fyuA+ was 42.3% there was no asn_RNA_intB gene. The results of serotype identifications showed that 15 strains related to serotype O4, O91, O78, O107, O38, O111, O88, O53, O24, O9 and O11 except 11 strains. Among 15 strains, O91, O78 and O38 were dominant serotypes whose proportion were 20 (3/15), 20 (3/15) and 26.7% (4/15), respectively. The nucleotide sequence similarities were >95% for 26 strains isolated from chickens.
Yanying Zhang, Qiumei Shi, Guisheng Gao, Hai Fang, Cuizhen Chen, Gaili Ren, Huoju Chang and Donglin Zhang, 2013. Phylogenetic Analysis and Detection of Related Genes of High-Pathogenicity Island of Escherichia coli Strains Isolated from Chicken in Hebei Province, China. Journal of Animal and Veterinary Advances, 12: 1177-1182.