Authors : Yang Cui-Jun, Ge Jian, Wu Shu-Qin, Chang Bao, Xu Tong and Wang Jin-Liang
Abstract: To produce Monoclonal Antibodies (McAb) against HA protein of H9 Avian Influenza Virus (AIV) and establish an Antigen Capture ELISA (AC-ELISA). BALB/c mice were immunized with HA recombination protein and the mouse splenic cells were fused with SP2/0 cells, hybridoma cell stably secreting anti-HA McAb was screened by ELISA coated with the AIV antigen an antigen capture ELISA was developed for detection of H9 AIV using polyclonal antibody as capture antibody and specific monoclonal antibody as detecting antibody. Hybridoma cell stably secreting anti-HA McAb designated 5F10, the immunoglobulin type of McAb identification shown was IgG2b type with κ chain. The H9 AIV AC-ELISA showed no cross-reaction with other five avian viruses (ILTV, NDV, EDSV, IBV and IBDV), the sensitivity eight times higher than HA test, comparing with RT-PCR, the concordance was 94.8%, the sensitivity was 97.1% and the specificity was 94.4%. The H9 AIV AC-ELISA has good specificity, sensitivity and repeatability could be used for diagnosing H9 subtype AIV infections.
Yang Cui-Jun, Ge Jian, Wu Shu-Qin, Chang Bao, Xu Tong and Wang Jin-Liang, 2013. Preparation of Monoclonal Antibodies Against HA Protein of H9 Subtype Avian Influenza Virus and Establishment Antigen Capture ELISA. Journal of Animal and Veterinary Advances, 12: 1651-1656.