Abstract: This study was designed to determine the dietary zinc requirement of Misgurnus anguillicaudatus by decision the effect of the graded levels of dietary zinc on growth, immune response and antioxidative. A total of 540 Misgurnus anguillicaudatus (initial weight 3.3±0.1 g) were randomly distributed into 6 groups of each three replicates, feeding diets containing graded levels of zinc (0, 15, 30, 60, 90 and 120 mg kg^-1 diet) for 80 days each group were sampled to measure weight gain, Specific Growth Rate (SGR), relative fatness and feed efficiency; protease, lipase and amylase activities in intestine and hepatopancreas the hago-rate erythrocyte, survival rate after vaccination, the Alkaline Phosphatase (AKP) and lysozyme activity in plasma; the Total Antioxidant enzymes (T-AOC), Catalase (CAT), Superoxide Dismutase (SOD), CuZn superoxide dismutase (CuZn-SOD) activities and Malondialdehyde (MDA) in plasma and hepatosomatic. Results indicate that the weight gain rate and SGR of dietary treatments were significantly the highest than group A. The group C was great significantly increased trypsin, lipase and amylase activities in intestine and hepatopancre. Zinc great significantly increased the hago-rate erythrocyte. AKP and lysozyme activity of group C in plasma were the highest than group A. The activities of T-AOC, CAT, SOD and CuZn-SOD of group C in plasma and hepatosomatic were great significantly increased than group A. The MDA of group C in plasma and hepatosomatic was great significantly decreased than group A. In summary, the optimal dietary zinc content for optimum growth performan, digestive ability and immune function in Misgurnus anguillicaudatus was about 30 mg kg^-1 of dietary zinc.