Authors : Shujie Wang, Liqin Li, Siguo Liu, Yonggang Liu, Yingfang Qi, Gang Wang, Yongqiang Jiang and Xuehui Cai
Abstract: Streptococcus suis (S. suis) secreted numerous proteins are virulence-associated factors that play critical roles in infection. Studies of S. suis secreted proteins have been reported in S. suis Serotype 2 (SS2) strains and S. suis Serotype 9 (SS9) strains. However, data is still scarce. In this study, a comparative proteomics approach was used to distinguish the profiles of the secreted proteins in different virulent SS7 strains. Consequently, 13 individual proteins that were unique to either high pathogenic 07WC11 strain or low pathogenic SJZB408 strain in 2D gels were successfully identified. In addition, researchers attempted to compare the amount of expression 10 identified protein genes (pft, rsp, cms, esp, hpk, pk, rpp, rrf, tef, tis) by quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR). Real-time RT-PCR results showed that pft, rsp, cms, esp, pk, rpp, rrf and tef mRNA expression amount in low pathogenic SS7 strain was lower than in high pathogenic SS7 strain which is accord with the results of 2D. Thus, researchers defined these eight proteins as different expression proteins between low pathogenic and high pathogenic SS7 strain.
Shujie Wang, Liqin Li, Siguo Liu, Yonggang Liu, Yingfang Qi, Gang Wang, Yongqiang Jiang and Xuehui Cai, 2013. Identification for Different Expression Proteins in Different Virulent Streptococcus suis Serotype 7 Strains. Journal of Animal and Veterinary Advances, 12: 533-538.