Authors : Chen Rui-Ai, Tang Xu, Wang Wenhao, Liu Haopeng, Zhang Xianhao and He Dongsheng
Abstract: This is the first study to construct successfully a hybrid protoplast of swine Escherichia coli (sw E. coli) E2010 with Hemophilus parasuis (Hps) H2011 strain. By conventional methods two types of bacteria, identified by specific drug resistant and rabbit-anti antibodies had been fused to construct a fusion protoplast named EH1011. EH1011 showed their parental drug resistance and agglutination identity by rabbit anti-E2010 serum and the rabbit anti-H2011 serum. The genetic characteristics of the fusion strains are stable within 15 generations through the identification of colony morphology, germ staining characteristics, biochemical characteristics and direct agglutination test. This fusion protoplast of swine E. coli and Hps has laid a foundation for the development of a new type of swine bivalent attenuated vaccine to control swine Escherichia coli and Hemophilus parasuis.
Chen Rui-Ai, Tang Xu, Wang Wenhao, Liu Haopeng, Zhang Xianhao and He Dongsheng, 2014. Construction of the Fusion Protoplast of Swine Escherichia coli with Hemophilus parasuis. Journal of Animal and Veterinary Advances, 13: 1-4.