Abstract: This study investigated the effects of different levels of vitamins A and E inclusion in diets on fecundity, egg fertilization and hatching rate in female brood fish of rainbow trout (Oncorhynchus mykiss, W.). In this rs of vitamin E (50, 100 ve 150 espects 7 diets including control diets (no vitamin A and E) containing two fixed amount of vitamin A with increasing doses of vitamin E (50, 100 ve 150 mg kg^-1 in diets containing fixed amount of vitamin A). Total number of 350 female and 70 male brood fish that had average mean body weight of 2-3 kg and 3+ aged stock were employed in this investigation. A total of 50 female and 10 male brood fish was stocked into each experimental concrete ponds. Investigation lasted in 70 days and randomly selected 15 female brood fish were spawned for eggs at the end of the study. There were significant (p<0.05) differences in terms of spawning performance among fish fed with different dietary treatments. However, it was evident that vitamin levels in diets did not affect the egg diameters but mean egg weights were significantly different in fish fed diets containing increased amount of vitamin E (p<0.05). Fertilized eggs were placed into egg trays and inspected throughout the investigation. It was also evident that diets containing increasing amount of vitamin E had significant (p<0.05) effect on the hatching rate of fertilized eggs and the survival of larvae during the incubation period. Findings of this study implied that it might be necessary to include sufficient amount of vitamin A and E in diets for rainbow trout for a successful fingerling production.