Authors : Wanyi Wang, Nagappan Mathialagan, Bernard Fermini and Weidong Hao
Abstract: An N-terminal truncated Androgen Receptor transcript variant 2 (AR2), lacking transcription activation domain has recently been identified in human and old world monkeys. The present study was designed to investigate whether corresponding AR2 variant mRNA is expressed in canine tissues. Alignment of human and canine genome AR sequences revealed 81% identity of the AR2 variant specific exon 1B in the canine genome sequence. A conserved 20 bp primer was designed for PCR amplification of canine AR2. By using Reverse Transcription-PCR analysis of RNA from multiple beagle dog tissues, researchers demonstrated the AR2 expression in dogs. Canine AR2 variantm RNA expression was observed in liver, kidney, heart and skeleton muscle suggesting a greater importance to investigate the role of AR2 in the physiological and pathological processes.
Wanyi Wang, Nagappan Mathialagan, Bernard Fermini and Weidong Hao, 2014. Expression Analysis of Androgen Receptor Transcript Variant 2 (AR2) in Beagle Dog Tissues. Journal of Animal and Veterinary Advances, 13: 165-170.