Authors : Xian Guo, Hongbo Wang, Jie Pei, Xuezhi Ding, Pengjia Bao, Chunnian Liang, Min Chu and Ping Yan
Abstract: The purpose of this study was to uncover differential patterns of mRNA expression for genes related to different development stages of yak (Bos grunniens) oocytes and preimplantation embryos. Researchers identified differentially expressed bands by mRNA differential display technology and evaluated the relative expression level of differentially expressed genes in oocytes, two cells, four cells and eight cells embryos and in morulae and blastulae. Researchers identified four differentially expressed bands of which one gene that was expressed during morula stage showed no homology to any other known genes in GenBank. Three other genes identified showed high identity with zinc finger MYND11 (ZMYND11), Nucleosome Assembly Protein 1-Like 1 (NAP1L1) and Ribosomal Protein L27A (RPL27A), respectively. The expression of ZMYND11 increased in mature oocytes, two cells and eight cells embryos and in the blastula with no significant differences between these stages (p>0.05). NAP1L1 was expressed at a relatively low level in mature oocytes, two cells embryos and in the blastula with a sharp increase in eight cells embryos (p<0.05). The expression of RPL27A decreased during development from mature oocytes to two cells embryos and then to eight cells stage embryos and increased significantly in cells of the blastula (p<0.05). ZMYND11, RPL27A and NAP1L1, the mRNA expression levels of which showed differences in different development stages of yak oocytes and early embryos might be related to normal development of early embryos and may be involved in maintaining normal reproductive function in yak.
Xian Guo, Hongbo Wang, Jie Pei, Xuezhi Ding, Pengjia Bao, Chunnian Liang, Min Chu and Ping Yan, 2014. Identification of Differentially Expressed Genes in Yak Preimplantation Embryos Derived from in vitro Fertilization. Journal of Animal and Veterinary Advances, 13: 197-202.