Authors : Wenqiang Sun, Liang Li, Jiwen Wang, Hehe Liu, Haohan Wang, Xinxin Li, Rongping Zhang and Fang Ding
Abstract: MyoD can bind the E-box sequence (CANNTG) in the promoters of downstream muscle target genes such as myogenin thereby regulating their transcription. The expression of myogenic regulatory factors is promoted by transcription of their corresponding genes and post-transcriptional regulation during which RNA-binding proteins have an important role. However, a post-transcriptional modulator of MyoD has not yet been reported. In the present study, a duck eukaryotic expression plasmid pEGFP-N1-MyoD was successfully constructed and expressed in duck myoblasts. After transfection, the number of cells and cellular myogenin expression were significantly increased. Furthermore, the expression of Rbm24 and Rbm38 mRNAs was significantly increased. These results suggest that overexpression of MyoD increased the expression of Rbm24 and Rbm38 during duck myoblast differentiation. These findings lay the foundations for further research of the post-transcriptional regulatory mechanisms of MyoD.
Wenqiang Sun, Liang Li, Jiwen Wang, Hehe Liu, Haohan Wang, Xinxin Li, Rongping Zhang and Fang Ding, 2014. Overexpression of MyoD Increased the Expression of RNA-Binding Proteins Rbm24 and Rbm38. Journal of Animal and Veterinary Advances, 13: 280-286.